7 on account of loss of water molecule. Actual molecular mass (M+ 402.0239, calc. 402.1804). It can be a neutral compound with no characteristic UV absorption. Its IR spectra (KBr) showed the presence of amide carbonyl (3400 cm-1, 1650 and 1639 cm-1) and mono-substituted phenyl moieties (1570, 755 and 720 cm-1) (17). The 1H NMR (CDCl3) showed the presence of these groups with ONH- signals at 6.61 (1H, d, J = 7.5 Hz) and 6.52 (1H, d, J = 7.5 Hz). The methylene group signals were amongst 2.90 and three.45. The 15 aromatic protons in a benzoyl (involving 7.48 ?7.68) with 3H generating multiplet and 2H creating doublet of doublet with J = 7, 2 Hz; Two benzyl of 5 protons in each group showing absorption at 7.20 and 7.39, br. One pair from the methylene protons aremagnetically equivalent even though the other folks were not resulting from exhibition of conformational rigidity imposed on the molecule by intramolecular hydrogen bonding on the methylene group (17). The diasterotopic methylene protons signal adjacent to hydroxyl was observed at 4.03 (1H, dd, J = 7.two, 6.four Hz) and 4.50(1H, dd, J = eight.29, five.38 Hz) (sub-structure (a)). The signals at four.61 (1H, m) and 4.19 (1H, dd, J = 4.28, four.03) have been assigned to methine proton next towards the diasterotopic methylene. The one hundred MHz 13C NMR spectra showed the presence of two carbonyl (167.4 and 167.2) in addition to 3 methylenes (65.4, 37.6 and 37.3) and two methines (54.5 and 50.3). The 65.4 was for major alcohol along with the homonuclear correlation between the diastereotropic protons adjacent to hydroxyl group at four.01 (C 65.four, HMQC) having a multiplet (1H) at four.61 which in turn revealed a correlation with a further diastereotropic benzylic proton at about 2.90, as a result confirming the sub-structure (a). Yet another benzylic diastereotropic proton at three.20 was revealed by COSY to correlate with proton at 4.92 (H). The down field shift of this proton supports sub-structure (b). The presence of a benzoyl group was strongly supported by the m/z 105 in the MS, giving the sub-structure (c). Aurantiamide acetate (2) colourless needle like crystals having a molecular ion peak m/z at 444.3016 for C27H28O4N2 (calc; 444.2205) by mass spectrometry. Its IR spectra indicated the presence of an ester carbonyl group (1735 cm-1) and amide groups (3410, 1670, 1653 and 1645 cm-1), whose presence was also supported by two protons signals (6.Isolation of Aurantiamides from Gomphrena Celosioides C. MartFigure two. Sub-structure for aurantiamide acetate.Methyl 1H-imidazole-5-carboxylate web and 5.5-Bromoimidazo[1,5-a]pyridine supplier 96) within the 1H NMR and by two amide carbon signals (167.PMID:23290930 1 and 170.2) inside the 13C NMR spectra. The homonuclear correlation 2D COSY (1H – 1H) experiments indicated the partial structures (d) and (e) together with unsubstituted benzoyl groups at 7.23 – 7.70. The acetyl was also determined through singlet methyl at H 2.02 ppm and its HMBC with carbonyl carbon C 170.2 ppm. The presence of these unsubstituted benzoyl and benzyl groups were also confirmed by the intense fragment ions at m/z 105 (87 ) and 91 (ten ) respectively. The 13C NMR spectra of aurantiamide acetate gave 27 signals as a result of two amide carbons, acetyl carbons (20.8 and 170.7), 18 aromatic carbons, three methylene carbons (37.5, 38.4 and 64.six), two methine carbons (49.5 and 54.9). Signal assignments were confirmed by a 2D- lengthy variety CH correlation (COLOG) experiment (18). The substitution of each and every group was confirmed by EI mass spectra, in which the fragment peaks at m/z 221 (21 ) and 252 (35 ) resulting in bond cleavage at either side from the central carbonyl gr.